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KMID : 1149220030110010115
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Korean Journal of Oriental Medical Prescription
2003 Volume.11 No. 1 p.115 ~ p.128
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Bojungikgitang Inhibits LPS Plus Interferon-γ-induced Inflammatory Mediators in RAW 264.7 Macrophages
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Jang Seon-Il
Kim Hyung-Jin
Kim Young-Jun
Pae Hyeon-Ok
Chung Hun-Taeg
Yun Yong-Gap
Jeong Ok-Sam
Kim Youn-Chul
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Abstract
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Bojungikgitang is the water extracts prepared from Ginseng Radix, Astragali Radix, Angelicae gigantis Radix, Astractylodis Rhizoma alba, Aurantii nobilis Pericarpium, Glycyrrhizae Radix, Bupleuri Radix, Cimicifugae Rhizoma, which has been used for the treatment of indigestion, and immunological disease in oriental countries. In this study, the effects of Bojungikgitang on the productions of nitiric oxide (NO) and prostaglandin E2(PGE2), and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were examined using RAW 264.7 macrophages activated with Interferon-γ(IFN-γ) plus lipopolysaccharide (LPS). Bojungikgitang (10-400 g/ml) per se had no cytotoxic effect in unstimulated macrophages, but this compound dose-dependently reduced the release of NO and PGE2 caused by stimulation of LPS/IFN-γ. The levels of iNOS and COX-2 protein were markedly suppressed by the treatment with Bojungikgitang in a concentration dependent manner. Moreover, Bojungikgitang also attenuated the production of tumor necrosis factor (TNF)-, interleukin (1L)-1β and IL-6 in LPS-stimulated RAW 264.7 macrophages. These results suggest that Bojungikgitang decreases the NO and PGE2 production in macrophages by inhibiting iNOS and COX-2 expression and these properties may contribute to the anti-inflammatory activity of Bojungikgitang.
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KEYWORD
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Bojungikgitang, nitric oxide, $prostaglandin\, E_2$, inducible nitric oxide synthase, cyclooxygenase-2
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